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Abstract Several agriculturally valuable plants store their pollen in tube-like poricidal anthers, which release pollen through buzz pollination. In this process, bees rapidly vibrate the anther using their indirect flight muscles. The stiffness and resonant frequency of the anther are crucial for effective pollen release, yet the impact of turgor pressure on these properties is not well understood. Here, we performed three-point flexure tests and experimental modal analysis to determine anther transverse stiffness and resonant frequency, respectively. Dynamic nanoindentation was used to identify the anther storage modulus as a function of excitation frequency. We subsequently developed mathematical models to estimate how turgor pressure changes after the anther is removed from a flower, thereby emulating zero water availability. We found that anther stiffness decreased by 60% at 30 min post-ablation and anther resonant frequency decreased by 20% at 60 min post-ablation. Models indicated that turgor pressure in the fresh anther was ~0.2–0.3 MPa. Our findings suggest that natural fluctuations in turgor pressure due to environmental factors such as temperature and light intensity may require bees to adjust their foraging behaviors. Interestingly, the anther storage modulus increased with excitation frequency, underscoring the need for more sophisticated mechanical models that consider viscous fluid transport through plant tissue.